Wayne I. Lencer, MD
Longwood Chair in Pediatrics, Harvard Medical School; Director, Harvard Digestive Disease Center
Longwood Professor of Pediatrics, Harvard Medical School
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Wayne I. Lencer, MD
Longwood Chair in Pediatrics, Harvard Medical School; Director, Harvard Digestive Disease Center
Longwood Professor of Pediatrics, Harvard Medical School
Medical Services
Languages
English
Education
Medical School
Boston University School of Medicine
1977
Boston
MA
Internship
Boston City Hospital
1978
Boston
MA
Residency
Boston City Hospital
1980
Boston
MA
Fellowship
Boston Children's Hospital
1987
Boston
MA
Certifications
American Board of Pediatrics (General)
Publications
The Genetic Architecture of Congenital Diarrhea and Enteropathy. View Abstract
Sorting of complex sphingolipids within the cellular endomembrane systems. View Abstract
SLO co-opts host cell glycosphingolipids to access cholesterol-rich lipid rafts for enhanced pore formation and cytotoxicity. View Abstract
Presentation of the 2024 AGA Distinguished Achievement Award in Basic Science to Jerrold R. Turner, MD, PhD, AGAF. View Abstract
Bacterial Sphingolipids Exacerbate Colitis by Inhibiting ILC3-derived IL-22 Production. View Abstract
IRE1a recognizes a structural motif in cholera toxin to activate an unfolded protein response. View Abstract
Protocol for measuring transcytosis and recycling of IgG in intestinal epithelial Caco-2 cells and primary human intestinal organoids. View Abstract
The epithelial-specific ER stress sensor ERN2/IRE1ß enables host-microbiota crosstalk to affect colon goblet cell development. View Abstract
Structural basis for acyl chain control over glycosphingolipid sorting and vesicular trafficking. View Abstract
Depletion of the apical endosome in response to viruses and bacterial toxins provides cell-autonomous host defense at mucosal surfaces. View Abstract
Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis. View Abstract
Cholera Toxin as a Probe for Membrane Biology. View Abstract
Small-molecule modulators of INAVA cytosolic condensate and cell-cell junction assemblies. View Abstract
Ceramide structure dictates glycosphingolipid nanodomain assembly and function. View Abstract
Evolution and function of the epithelial cell-specific ER stress sensor IRE1ß. View Abstract
Extracellular cyclic dinucleotides induce polarized responses in barrier epithelial cells by adenosine signaling. View Abstract
A Quantitative Single-cell Flow Cytometry Assay for Retrograde MembraneTrafficking Using Engineered Cholera Toxin. View Abstract
Everything Illuminated-Clostridium perfringens ß-toxin. View Abstract
Structured clustering of the glycosphingolipid GM1 is required for membrane curvature induced by cholera toxin. View Abstract
Conjugation of peptides to short-acyl-chain ceramides for delivery across mucosal cell barriers. View Abstract
IRE1ß negatively regulates IRE1a signaling in response to endoplasmic reticulum stress. View Abstract
A quantitative single-cell assay for retrograde membrane traffic enables rapid detection of defects in cellular organization. View Abstract
A modified cholera toxin B subunit containing an ER retention motif enhances colon epithelial repair via an unfolded protein response. View Abstract
INAVA-ARNO complexes bridge mucosal barrier function with inflammatory signaling. View Abstract
Transcytosis Assay for Transport of Glycosphingolipids across MDCK-II Cells. View Abstract
Transcytosis Assay for Transport of Glycosphingolipids across MDCK-II Cells. View Abstract
CRISPR Screen Reveals that EHEC's T3SS and Shiga Toxin Rely on Shared Host Factors for Infection. View Abstract
Mucosal absorption of therapeutic peptides by harnessing the endogenous sorting of glycosphingolipids. View Abstract
Advances in Evaluation of Chronic Diarrhea in Infants. View Abstract
Retraction Notice to: The Unfolded Protein Response Element IRE1a Senses Bacterial Proteins Invading the ER to Activate RIG-I and Innate Immune Signaling. View Abstract
Membrane Transport across Polarized Epithelia. View Abstract
Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury. View Abstract
A targeted RNAi screen identifies factors affecting diverse stages of receptor-mediated transcytosis. View Abstract
Aquaporin-3 mediates hydrogen peroxide-dependent responses to environmental stress in colonic epithelia. View Abstract
Glycolipid Crosslinking Is Required for Cholera Toxin to Partition Into and Stabilize Ordered Domains. View Abstract
CellMapper: rapid and accurate inference of gene expression in difficult-to-isolate cell types. View Abstract
Opening CFTR in the Intestine: Flushing on Demand. View Abstract
Microbial sphingomyelinase induces RhoA-mediated reorganization of the apical brush border membrane and is protective against invasion. View Abstract
Electrophysiological Studies into the Safety of the Anti-diarrheal Drug Clotrimazole during Oral Rehydration Therapy. View Abstract
Congenital chloride-losing diarrhea in a Mexican child with the novel homozygous SLC26A3 mutation G393W. View Abstract
Innate immunity at mucosal surfaces: the IRE1-RIDD-RIG-I pathway. View Abstract
FcRn: The Architect Behind the Immune and Nonimmune Functions of IgG and Albumin. View Abstract
Microtubule motors power plasma membrane tubulation in clathrin-independent endocytosis. View Abstract
4-Phenylbutyrate attenuates the ER stress response and cyclic AMP accumulation in DYT1 dystonia cell models. View Abstract
Remodeling of the intestinal brush border underlies adhesion and virulence of an enteric pathogen. View Abstract
Mechanism for adhesion G protein-coupled receptor GPR56-mediated RhoA activation induced by collagen III stimulation. View Abstract
Unsaturated glycoceramides as molecular carriers for mucosal drug delivery of GLP-1. View Abstract
Neonatal Fc receptor expression in dendritic cells mediates protective immunity against colorectal cancer. View Abstract
Fc-fusion proteins and FcRn: structural insights for longer-lasting and more effective therapeutics. View Abstract
Ganglioside GM1-mediated transcytosis of cholera toxin bypasses the retrograde pathway and depends on the structure of the ceramide domain. View Abstract
Multivalent immune complexes divert FcRn to lysosomes by exclusion from recycling sorting tubules. View Abstract
The unfolded protein response element IRE1a senses bacterial proteins invading the ER to activate RIG-I and innate immune signaling. View Abstract
A single native ganglioside GM1-binding site is sufficient for cholera toxin to bind to cells and complete the intoxication pathway. View Abstract
Lipid sorting by ceramide structure from plasma membrane to ER for the cholera toxin receptor ganglioside GM1. View Abstract
The immunologic functions of the neonatal Fc receptor for IgG. View Abstract
Cross-presentation of IgG-containing immune complexes. View Abstract
Insights on the trafficking and retro-translocation of glycosphingolipid-binding bacterial toxins. View Abstract
Eliciting mucosal immunity. View Abstract
To translocate or not: that is the problem. View Abstract
Discovery of dual-action membrane-anchored modulators of incretin receptors. View Abstract
Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes. View Abstract
TorsinA participates in endoplasmic reticulum-associated degradation. View Abstract
Neonatal Fc receptor for IgG (FcRn) regulates cross-presentation of IgG immune complexes by CD8-CD11b+ dendritic cells. View Abstract
The zebrafish dag1 mutant: a novel genetic model for dystroglycanopathies. View Abstract
Functional analysis of VopF activity required for colonization in Vibrio cholerae. View Abstract
Intoxication of zebrafish and mammalian cells by cholera toxin depends on the flotillin/reggie proteins but not Derlin-1 or -2. View Abstract
Neonatal Fc receptor: from immunity to therapeutics. View Abstract
Cholera toxin: an intracellular journey into the cytosol by way of the endoplasmic reticulum. View Abstract
Selective translocation of the Bordetella pertussis adenylate cyclase toxin across the basolateral membranes of polarized epithelial cells. View Abstract
N-terminal extension of the cholera toxin A1-chain causes rapid degradation after retrotranslocation from endoplasmic reticulum to cytosol. View Abstract
An FcRn-dependent role for anti-flagellin immunoglobulin G in pathogenesis of colitis in mice. View Abstract
Immune and non-immune functions of the (not so) neonatal Fc receptor, FcRn. View Abstract
The recycling and transcytotic pathways for IgG transport by FcRn are distinct and display an inherent polarity. View Abstract
N-Glycan Moieties in Neonatal Fc Receptor Determine Steady-state Membrane Distribution and Directional Transport of IgG. View Abstract
Ceramide activates JNK to inhibit a cAMP-gated K+ conductance and Cl- secretion in intestinal epithelia. View Abstract
Patching a leaky intestine. View Abstract
Dependence of antibody-mediated presentation of antigen on FcRn. View Abstract
Attenuated endocytosis and toxicity of a mutant cholera toxin with decreased ability to cluster ganglioside GM1 molecules. View Abstract
Conversion of apical plasma membrane sphingomyelin to ceramide attenuates the intoxication of host cells by cholera toxin. View Abstract
Derlin-1 facilitates the retro-translocation of cholera toxin. View Abstract
Ca2+-dependent calmodulin binding to FcRn affects immunoglobulin G transport in the transcytotic pathway. View Abstract
The HA proteins of botulinum toxin disrupt intestinal epithelial intercellular junctions to increase toxin absorption. View Abstract
Rafting with cholera toxin: endocytosis and trafficking from plasma membrane to ER. View Abstract
How the controller is controlled - neonatal Fc receptor expression and immunoglobulin G homeostasis. View Abstract
IgG transport across mucosal barriers by neonatal Fc receptor for IgG and mucosal immunity. View Abstract
Neonatal Fc receptor for IgG regulates mucosal immune responses to luminal bacteria. View Abstract
Protein disulfide isomerase-like proteins play opposing roles during retrotranslocation. View Abstract
Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure. View Abstract
The Cdc42 inhibitor secramine B prevents cAMP-induced K+ conductance in intestinal epithelial cells. View Abstract
The viral E3 ubiquitin ligase mK3 uses the Derlin/p97 endoplasmic reticulum-associated degradation pathway to mediate down-regulation of major histocompatibility complex class I proteins. View Abstract
A biochemical method for tracking cholera toxin transport from plasma membrane to Golgi and endoplasmic reticulum. View Abstract
Antibodies in the breakdown lane. View Abstract
Raft trafficking of AB5 subunit bacterial toxins. View Abstract
Calnexin and ERp57 facilitate the assembly of the neonatal Fc receptor for IgG with beta 2-microglobulin in the endoplasmic reticulum. View Abstract
Role of p97 AAA-ATPase in the retrotranslocation of the cholera toxin A1 chain, a non-ubiquitinated substrate. View Abstract
Characterization of the porcine neonatal Fc receptor--potential use for trans-epithelial protein delivery. View Abstract
Signal transduction by bacterial proteins. View Abstract
Endocytosis of cholera toxin by human enterocytes is developmentally regulated. View Abstract
Entry of protein toxins into mammalian cells by crossing the endoplasmic reticulum membrane: co-opting basic mechanisms of endoplasmic reticulum-associated degradation. View Abstract
Ganglioside GD1a restores infectibility to mouse cells lacking functional receptors for polyomavirus. View Abstract
A passionate kiss, then run: exocytosis and recycling of IgG by FcRn. View Abstract
Trafficking of cholera toxin-ganglioside GM1 complex into Golgi and induction of toxicity depend on actin cytoskeleton. View Abstract
Pulmonary delivery of an erythropoietin Fc fusion protein in non-human primates through an immunoglobulin transport pathway. View Abstract
Human neonatal Fc receptor mediates transport of IgG into luminal secretions for delivery of antigens to mucosal dendritic cells. View Abstract
Retrograde transport of cholera toxin from the plasma membrane to the endoplasmic reticulum requires the trans-Golgi network but not the Golgi apparatus in Exo2-treated cells. View Abstract
Cholera toxin toxicity does not require functional Arf6- and dynamin-dependent endocytic pathways. View Abstract
Retrograde transport of cholera toxin into the ER of host cells. View Abstract
Paneth cell cryptdins act in vitro as apical paracrine regulators of the innate inflammatory response. View Abstract
Bidirectional transepithelial IgG transport by a strongly polarized basolateral membrane Fcgamma-receptor. View Abstract
Diarrhea-associated HIV-1 APIs potentiate muscarinic activation of Cl- secretion by T84 cells via prolongation of cytosolic Ca2+ signaling. View Abstract
The intracellular voyage of cholera toxin: going retro. View Abstract
Gangliosides that associate with lipid rafts mediate transport of cholera and related toxins from the plasma membrane to endoplasmic reticulm. View Abstract
Gangliosides are receptors for murine polyoma virus and SV40. View Abstract
Claudin-8 expression in Madin-Darby canine kidney cells augments the paracellular barrier to cation permeation. View Abstract
Distribution of the IgG Fc receptor, FcRn, in the human fetal intestine. View Abstract
Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation. View Abstract
The heavy chain of neonatal Fc receptor for IgG is sequestered in endoplasmic reticulum by forming oligomers in the absence of beta2-microglobulin association. View Abstract
Receptor-mediated immunoglobulin G transport across mucosal barriers in adult life: functional expression of FcRn in the mammalian lung. View Abstract
Functional reconstitution of human FcRn in Madin-Darby canine kidney cells requires co-expressed human beta 2-microglobulin. View Abstract
Cryptdin 3 forms anion selective channels in cytoplasmic membranes of human embryonic kidney cells. View Abstract
Uncoupling of the cholera toxin-G(M1) ganglioside receptor complex from endocytosis, retrograde Golgi trafficking, and downstream signal transduction by depletion of membrane cholesterol. View Abstract
Research agenda for pediatric gastroenterology, hepatology and nutrition: secretion and diarrhea. Report of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition for the Children's Digestive Health and Nutrition Foundation. View Abstract
Characterization of receptor-mediated signal transduction by Escherichia coli type IIa heat-labile enterotoxin in the polarized human intestinal cell line T84. View Abstract
Vibrio cholerae-induced cellular responses of polarized T84 intestinal epithelial cells are dependent on production of cholera toxin and the RTX toxin. View Abstract
A cholera toxin B-subunit variant that binds ganglioside G(M1) but fails to induce toxicity. View Abstract
A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity. View Abstract
The multiple roles of major histocompatibility complex class-I-like molecules in mucosal immune function. View Abstract
Microbes and microbial Toxins: paradigms for microbial-mucosal toxins. V. Cholera: invasion of the intestinal epithelial barrier by a stably folded protein toxin. View Abstract
Protein disulfide isomerase acts as a redox-dependent chaperone to unfold cholera toxin. View Abstract
MHC class I-related neonatal Fc receptor for IgG is functionally expressed in monocytes, intestinal macrophages, and dendritic cells. View Abstract
Cryptdin-3 induces novel apical conductance(s) in Cl- secretory, including cystic fibrosis, epithelia. View Abstract
Microbial invasion across the intestinal epithelial barrier. View Abstract
Association of protease activity in Vibrio cholerae vaccine strains with decreases in transcellular epithelial resistance of polarized T84 intestinal epithelial cells. View Abstract
Floating cholera toxin into epithelial cells: functional association with caveolae-like detergent-insoluble membrane microdomains. View Abstract
Heterogeneity of detergent-insoluble membranes from human intestine containing caveolin-1 and ganglioside G(M1). View Abstract
Salmonella pathogenesis: the trojan horse or the New York shuttle? View Abstract
Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line. View Abstract
Floating the molecular machinery for t-cell activation. View Abstract
Membrane traffic and the cellular uptake of cholera toxin. View Abstract
Antigen presentation by intestinal epithelial cells. View Abstract
Anthrax toxin entry into polarized epithelial cells. View Abstract
Structural basis for the differential toxicity of cholera toxin and Escherichia coli heat-labile enterotoxin. Construction of hybrid toxins identifies the A2-domain as the determinant of differential toxicity. View Abstract
Mucosal vaccination: "all politics are local". View Abstract
The potassium channel and how it works. View Abstract
Differential activity of cholera toxin and E. coli enterotoxin: construction and purification of mutant and hybrid derivatives. View Abstract
Distinct Ca2+- and cAMP-dependent anion conductances in the apical membrane of polarized T84 cells. View Abstract
Paneth cells: on the front line or in the backfield? View Abstract
Ganglioside structure dictates signal transduction by cholera toxin and association with caveolae-like membrane domains in polarized epithelia. View Abstract
Acid secretion, ulcer disease, and cell biology. View Abstract
The antifungal antibiotic, clotrimazole, inhibits chloride secretion by human intestinal T84 cells via blockade of distinct basolateral K+ conductances. Demonstration of efficacy in intact rabbit colon and in an in vivo mouse model of cholera. View Abstract
Induction of epithelial chloride secretion by channel-forming cryptdins 2 and 3. View Abstract
Proteolytic activation of cholera toxin and Escherichia coli labile toxin by entry into host epithelial cells. Signal transduction by a protease-resistant toxin variant. View Abstract
Surface expression, polarization, and functional significance of CD73 in human intestinal epithelia. View Abstract
The antifungal antibiotic, clotrimazole, inhibits Cl- secretion by polarized monolayers of human colonic epithelial cells. View Abstract
Role of the glycocalyx in regulating access of microparticles to apical plasma membranes of intestinal epithelial cells: implications for microbial attachment and oral vaccine targeting. View Abstract
Carboxy-terminal vesicular stomatitis virus G protein-tagged intestinal Na+-dependent glucose cotransporter (SGLT1): maintenance of surface expression and global transport function with selective perturbation of transport kinetics and polarized expression. View Abstract
Targeting of cholera toxin and Escherichia coli heat labile toxin in polarized epithelia: role of COOH-terminal KDEL. View Abstract
Transcytosis of cholera toxin subunits across model human intestinal epithelia. View Abstract
Signal transduction by cholera toxin: processing in vesicular compartments does not require acidification. View Abstract
The A2b adenosine receptor mediates cAMP responses to adenosine receptor agonists in human intestinal epithelia. View Abstract
Bacterial and host interactions during the biogenesis, toxicity and immunogenicity of Escherichia coli heat-labile enterotoxin. View Abstract
Heterologous expression of delta F508 CFTR results in decreased sialylation of membrane glycoconjugates. View Abstract
Monoclonal immunoglobulin A antibodies directed against cholera toxin prevent the toxin-induced chloride secretory response and block toxin binding to intestinal epithelial cells in vitro. View Abstract
Entry of cholera toxin into polarized human intestinal epithelial cells. Identification of an early brefeldin A sensitive event required for A1-peptide generation. View Abstract
Mechanism of cholera toxin action on a polarized human intestinal epithelial cell line: role of vesicular traffic. View Abstract
Cl- secretion in a model intestinal epithelium induced by a neutrophil-derived secretagogue. View Abstract
Endocytosis of water channels in rat kidney: cell specificity and correlation with in vivo antidiuresis. View Abstract
Endocytic vesicles from renal papilla which retrieve the vasopressin-sensitive water channel do not contain a functional H+ ATPase. View Abstract
[Characterization of proton pump and osmotic water transport in endocytic vesicles from rat kidney proximal tubule]. View Abstract
FITC-dextran as a probe for endosome function and localization in kidney. View Abstract
Interaction of cholera toxin with cloned human goblet cells in monolayer culture. View Abstract
Functional colocalization of water channels and proton pumps in endosomes from kidney proximal tubule. View Abstract
Endosomes from kidney collecting tubule cells contain the vasopressin-sensitive water channel. View Abstract
Differential binding kinetics of cholera toxin to intestinal microvillus membrane during development. View Abstract
Unionization of house staff. View Abstract